摘要
Genetic diversity of Dendrolimus punctatus over five regions in Zhejiang Province were studied by ISSR-PCR.Thirteen primers were screened on the template of total DNA of the insects and total 74 bands of DNA fragments were amplified,of which the half(37 bands)was polymorphic.The genetic distance among the sampled populations ranged 0.114 4-0.475 4.The dendrogram was constructed based on their genetic distance using UPGMA method.The results indicated that there was low genetic diversity among the four populations from the main land,while the Dongtou population sampled from Dongtou island showed higher genetic diversity.
Genetic diversity of Dendrolimus punctatus over five regions in Zhejiang Province were studied by ISSR - PCR. Thirteen primers were screened on the template of total DNA of the insects and total 74 bands of DNA fragments were amplified, of which the half （37 bands） was polymorphic. The genetic distance among the sampled populations ranged 0.114 4 ～ 0.475 4. The dendrogram was constructed based on their genetic distance using UPGMA method. The results indicated that there was low genetic diversity among the four populations from the main land, while the Dongtou population sampled from Dongtou island showed higher genetic diversity.
出处
《林业科学》
EI
CAS
CSCD
北大核心
2008年第1期164-168,共5页
Scientia Silvae Sinicae
基金
浙江省科技计划重点项目(2004C22037)资助
关键词
马尾松毛虫
ISSR
遗传差异
多态性
Dendrolimus punctatus
ISSR
genetic diversity
polymorphic
