日本落叶松同工酶变异的基因分析及无性系鉴别

The Gene Analysis of Isozyme Variation and its Clone Identification in Larix Kaempferi

同工酸可作为遗传图谱构建和无性系鉴别的重要遗传标记．以92个日本落叶松无性系种子为实验材料，采用垂直平板聚丙烯酰胺凝胶电泳法，取其胚乳进行了同工酶分析，结果表明：11种酶系统至少由24个基因座所控制，多数座位存在2个或2个以上的等位基因；多数座位等位基因的基因频率差异很大；各无性系之间至少在一个以上的基因座上存在差异．并确定了92个无性系的同工酶基因型．

Isozyme can be used as an important genetic marker for the construction of agenetic map and for clone identification. By conducting experiments on the endosperms ofninty-two Larix kaemPfri clone seeds, ninty-two cl0ne isozyme geneotypes have been de-termined. Eleven isozyme systems are controlled by twenty-four loci, most of which havetwo or more alleles each. The gene frequencies of these alleles differ greatly. Even everyclone differs from the other at least in one locus.