血管内皮生长因子和血小板反应素在卵巢上皮癌中的表达及其意义

Expression of vascular endothelial growth factor and thrombospodinl in epithelial ovarian cancer and their correlations with tumor angiogenesis and prognosis

目的探讨测血管内皮生长因子（VEGF）和血小板反应素1（TSP1）蛋白质在卵巢上皮癌中的表达与肿瘤微血管密度（MVD）的关系及其临床意义。方法用免疫组织化学法检测57例原发性卵巢上皮癌组织和22例正常卵巢组织中VEGF和TSP1蛋白质的表达，并用CD34抗体免疫染色后进行MVD计数。结合随诊资料，对上述指标与卵巢上皮癌临床病理学特征及3年存活率之间的关系进行回顾分析。结果卵巢上皮癌组织中VEGF蛋白质的阳性表达率高于正常卵巢组织（分别为70．2％和45．5％，P〈0．05），且多为强阳性表达。卵巢上皮癌中组织中TSP1多为弱阳性表达，总阳性表达率低于正常卵巢组织（分别为47．4％和72．7％，P〈0．05）。卵巢上皮癌组织的MVD显著高于正常卵巢组织（分别为30士9和20士8，P〈0．05）。VEGF阳性和TSP1阴性与卵巢上皮癌的手术病理学分期晚、大网膜转移和癌性腹水显著相关。VEGF的表达与MVD呈正相关（P〈0．01），TSP1的表达与MVD呈负相关（P〈0．01）。VEGF阳性和TSP1阴性均与患者3年存活率呈负相关。VEGF阳性而TSP1阴性组的3年存活率明显低于VEGF阴性而TSP1阳性组（分别为24．0％和83．3％，P〈0．01）。结论卵巢上皮癌组织VEGF和TSP1蛋白质的表达与肿瘤的血管形成有关，对上述分子的检测可帮助判断卵巢上皮癌的生物学行为，从而提供预后信息。

Objective To evaluate the expression of vascular endothelial growth factor （VEGF） and thrombospodinl （TSP1） in epithelial ovarian cancer and their correlations with tumor angiogenesis and prognosis. Methods Fifty-seven samples of primary epithelial ovarian cancer and 22 samples of normal ovary from patients with hysteromyoma obtained during operation underwent immunohistochemistry with anti- VEGF, anti-TSP1, and anti-CD34 antibodies. Microvessel density （MVD） in the tumor tissue was evaluated on the base of CD-34 immunostaining. The correlations of VEGF and TSP1 expression with clinico-pathologic characteristics and 3-years survival rates were analyzed retrospectively. Results The positive rate of VEGF protein of the epithelial ovarian cancer was 70.2%, significantly higher than that of the normal ovarian tissue （45.5%, P 〈 0.05 ）. The positive rate of TSP1 protein of the epithelial ovarian cancer was 47.4%, significantly lower than that of the normal ovarian tissue （ 72.7%, P 〈 0.05 ）. The MVD of the epithelial ovarian cancer was （30 ± 9）, significantly higher than that of the normal ovarian tissue （ 20 ± 8, P 〈 0. 05 ）. The VEGF positive expression and TSP1 negative expression were positively correlated with the FIGO stage, omentum metastases, and malignant ascites （ P 〈 0. 01, 〈 0. 01, and 〈 0. 05 respectively）. VEGF expression was positively correlated with MVD （ P 〈 0.01 ）, and TSP1 expression was negatively correlated with MVD （ P 〈 0.01 ）. Increased expression of VEGF and decreased expression of TSP1 with were reversely correlated with the patients＇ survival. The 3-year survival rate of the patients positive in VEGF and negative in TSP1 was 24.0%, significantly lower than that of the patients negative in VEGF and positive in TSP1 （83.3%, P 〈 0.01 ）. Conclusion Increased expression of VEGF and decreased expression of TSP1 in epithelial ovarian cancer are closely related to its angiogenesis. Detection of these two proteins contributes to identifying their biological behavior and prognosis.