动脉栓塞化疗对肾母细胞瘤细胞增殖的影响

Effect of preoperative transcatherter arterial chemo-embolization on activity of cell proliferation in Wilms' tumor

目的:探讨动脉栓塞化疗对肾母细胞瘤肿瘤细胞退变及增殖细胞核抗原(PCNA)和血管内皮生长因子(VEGF)表达的影响。方法:将41例经病理确诊的肾母细胞瘤患儿分成两组,动脉栓塞化疗(TACE)组23例,先进行TACE治疗,2周后再行肿瘤切除;对照组18例,直接行肿瘤切除。应用常规染色和免疫组化法染色,观察肾母细胞瘤细胞增殖的影响。结果:术前进行动脉栓塞化疗后的肾母细胞瘤细胞退变程度明显高于对照组,增殖细胞核抗原的表达指数阳性率明显低于对照组(4.3%vs 50%,P<0.01),而VEGF的阳性表达则TACE组与对照组无显著性差异(P>0.05)。结论:动脉栓塞化疗可加剧肾母细胞瘤肿瘤细胞的退变,对肿瘤细胞的增殖有明显的抑制作用,而与血管内皮生长因子表达无相关性。

Objective： To investigate the effect of preoperative transcatherter arterial chemoembolization （TACE） on the cell proliferation in Wilms＇ tumor. Methods： Forty-one cases of Wilms＇ tumor diagnosed by histopathology were divided into two groups：in TACE group, 23 patients received TACE first and were operated 2 weeks later ; in control group, 18 patients were operated alone. A comparative analysis of the pathological finding was made in two groups,and the expression of PCNA and VEGF in tumor tissue was detected by immunohistochemistry. Results： The degeneration of tumor tissue such as tumor cell necrotic, broken, disappearance occurred in 17 cases of TACE group and in 4 cases of control group,respectively （P〈0.01）. The expression of PCNA in TACE group and in control group was 1/23（4. 3%） and 9/18（50.0%）, respectively （P〈0. 01）. VEGF was expressed in 7/23（30.4%） of TACE group and 9/18 （50.0%） of control group （P=0. 283）. Conclusion： TACE can significantly inhibit proliferation and enhance degeneration of Wilms＇ tumor cells.