人胚胎干细胞建系及其研究进展

Progress in the derivation,culture of human embryonic stem cells

人胚胎干细胞(human embryonic stem cell,hES)在再生医学、药物筛选和发育生物学等领域具有重要的研究和应用价值。hES细胞系通常生长在小鼠胚胎成纤维细胞上,动物源性病原生物的污染风险限制了这类hES的临床应用,因此寻找无血清、无饲养层细胞的成分明确的培养体系进行hES细胞建系及培养,生产细胞移植临床安全应用标准的hES是近年hES建系的努力方向。

Human embryonic stem （hES） cells are considered to be a valuable resource for research in regenerative medicine,drug screening,and developmental studies. However,hES cells are usually established and maintained on mouse embryonic fibrohlast feeder layers,and the risk of animal origin contamination from feeder layer generally excludes the clinical use of these hES cells. The main emphasis over the last several years has been in finding defined serum- and feeder layer-free system for derivation and culture of hES cells to enable the clinical use of hES cell for cell transplantation.