摘要
HBsAg抗原145位突变,可能导致临床上广泛使用的HBsAg诊断试剂出现漏检.通过杂交瘤技术制备出了一种抗该突变体的单克隆抗体,属IgG1亚类(κ型,效价为1∶9×104)与其他类型肝炎病毒抗原无交叉反应.采用该单抗与多克隆抗体所建立的AC-ELISA法,对临床上乙型肝炎病毒(HBV)的突变样品进行检测的特异性和敏感性较高.
Hepatitis B virus surface antigen with mutation on site 145 may cause omission in detection of hepatitis B virus with HBsAg diagnostic reagent. Monoclonal antibody against this mutation was obtained through hybridoma technique. The sub-class of the monoclonal antibody was determined to be IgG1 (κ type, the titer is 1 : 9×10^4), and the monoclonal antibody has no cross-reaction to other hepatitis virus. The method of AC-ELISA which was established with monoclonal antibody and polyclonal antibody showed high analysing susceptibility for HBV mutant samples.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
2008年第2期231-234,共4页
Progress In Biochemistry and Biophysics
基金
吉林省科技发展计划项目(20060923-02)
长春市科技局资助项目(2005-120/05GG76)~~