高剂量三氯乙烯对L-02肝细胞中Rho GDIα表达的影响

Rho GDIα Expression Change in L-02 Liver Cells after Treatment with High Concentration of Trichloroethylene

背景与目的:观察高剂量三氯乙烯(trichloroethylene,TCE)对L-02肝细胞中Rho GDIα基因在mRNA及蛋白水平表达的影响。材料与方法:以β-actin为内参,采用蛋白印迹(western blot)和实时荧光定量RT-PCR技术对分别40μmol/L TCE(实验组)和DMSO(溶剂对照组)处理的正常L-02肝细胞中的Rho GDIα蛋白和mRNA水平进行检测。结果:高剂量TCE处理后Rho GDIαmRNA表达量显著下降而蛋白含量明显升高。结论:Rho GDIα基因表达在高剂量TCE处理后发生了特异性改变,为揭示TCE对机体的损伤作用机制提供了重要线索。

BACKGROUND AND AIM： To study the effect of trichloroethylene（TCE） on the expression change of Rho GDla gene at both mRNA and protein levels. MATERIALS AND METHODS： Western blot and real time quantitative RT-PCR were employed to detect the protein and mRNA levels of Rho GDla in L-02 liver cells ,which were treated with DMSO and high concentrations of TCE.β-actin was selected as internal standard. RESULTS： The protein level of Rho GDIα was significantly up-regulated while its mRNA was significantly reduced. CONCLUSION： The Rho GDla gene expression level showed specific changes after treatment with high concentration of TCE, providing important clues to the mechanism of TCE-induced damage in humans.