木瓜苷的致畸毒性研究

Study on Teratogenicity of Glycosides of Chaenomeles Speciossa

背景与目的:评价木瓜苷对ICR小鼠的胚胎毒性和致畸毒性,为其临床应用提供毒理学依据。材料与方法:ICR孕鼠随机分为5组:木瓜苷3个剂量组(83.1、332.5、1330.0mg/kg),阴性对照组[0.5%羧甲基纤维素钠(CMC-Na)]及阳性对照组[20mg/kg环磷酰胺(CP)]。阳性对照组于孕第10d肌肉一次注射CP0.01ml/g,其余各组均为孕第6～15d(胚胎器官形成期)灌胃给予0.02ml/g木瓜苷或CMC-Na,每组大于15只。在妊娠的第18d,颈椎脱臼处死,计数黄体数、胚胎着床数、活胎数、死胎数和吸收胎数;观察胎仔外观、性别并称量其体重后,将每窝1/2的活胎仔乙醇固定、2%氢氧化钾软化、茜素红染色、甘油透明后检查骨骼发育情况。另1/2活胎仔经Bouin’s液固定后,检查内脏发育情况。结果:在实验剂量范围内,木瓜苷各剂量组孕鼠的生殖能力、胚胎形成和胎仔外观、骨骼及内脏生长发育与阴性对照组相比差异均无统计学意义(P均>0.05),但木瓜苷1330.0mg/kg剂量组孕鼠总增重[(4.97±1.53)g]与阴性对照组[(6.59±1.37)g]相比明显降低,差异具有统计学意义(P<0.05)。结论:本实验条件下木瓜苷对小鼠无胚胎毒性和致畸毒性。

BACKGROUND AND AIM： To study teratogenicity of glycosides of chaenomeles speciossa （GCS） on ICR mice during the sensitive period. MATERIALS AND METHODS： The pregnant ICR mice were divided into five groups in this test, GSC groups （83.1, 332.5, 1330.0 mg/kg） , one positive control group （CP） and one negative control group （0.5%CMC-Na） Every group consisted of 15 pregnant mice at least .The mice of the GCS groups and the negative group were treated with 0.02 ml/g orally during the period of organ formation（from Day 6 to Day 15,once a day）,while the positive control mice received 0.01 ml/g intramuscular injection on Day 10. The pregnant mice were killed by cervical vertebral dislocation on Day 18, then the numbers of corpus lutea, living embryo, dead fetus and absorbed fetus were counted, the sex and appearance of the mice embryos were examined, tail length and weight were measured .After that half of living embryos of every pregnant mouse were used to study the bone development after ethanol fixing, potassium hydroxide melting, Alizarin Red staining and glycerin transparencing. The other living embryos were examined for the visceral development after Bouin＇s solution fixing. RKSULTS： Compared with the negative control group, no abnormalities in the living embryos appearance, bones and viscera were found in any of the three dosages groups, while inatemal body weight of highest dosage of GCS（1 330.0 mg/ kg） increased slowly. CONCLUSION： GCS（83.1- 1 330.0 mg/kg） had no embryonic and teratogenic toxicity in this test.