异质性胞核核糖核蛋白K在肝癌组织及不同密度肝癌细胞中的表达

Expression of heterogeneous nuclear ribonucleoprotein K in hepatocellular carcinoma tissues and cell line of different cell densities

目的:观察异质性胞核核糖核蛋白K(heterogeneous nuclear ribonucleoprotein K,hnRNPK)在肝细胞癌(hepatocellular carcinoma,HCC)组织中的表达及在不同密度肝癌细胞(PLC/PRF/5)中的亚细胞定位。方法:免疫组化染色观察肝细胞癌组织中hnRNPK的表达;Western印迹检测肝癌组织(n=30)及对应癌旁组织hnRNPK蛋白表达差异;应用细胞免疫组化和Western印迹法比较不同密度肝癌细胞hnRNPK蛋白的亚细胞定位及表达。结果:肝细胞癌组织hnRNPK蛋白主要表达于细胞核。hnRNPK蛋白表达在30例的肝癌组织中有21例高于其对应的癌旁组织;20倒HBV阳性肝癌组织有16例高于其对应的癌旁组织,而10例HBV阴性肝癌组织仅有5例表达高于癌旁组织。细胞免疫组化结果表明。hnRNPK主要表达于PLC细胞核中,并且随密度(汇合率为30%、50%、90%)的上升。其表达量逐渐上升。Western印迹结果表明,不同密度(汇合率为30%、50%、70%、90%)肝癌细胞的细胞质中,hnRNPK的表达量无统计学差异;而在细胞核中,随着细胞密度的增大,hnRNPK的表达量逐渐升高。结论:肝癌组织细胞核hnRNPK表达上调,且随着肝癌细胞密度的增加。其表达会逐渐增强,HBV感染可能会促进其表达。

Objective：To investigate the expression changes and the subcellular localization of heterogeneous nuclear ribonu- cleoprotein K （hnRNP K） in hepatocellular carcinoma （HCC） and cell line of different cell densities. Methods： The expression of hnRNP K in HCC was detected by immunohistochemical staining. The differential expression of hnRNP K in the HCC tissue （n=30） and paired paracancerous tissue was identified by Western blotting assay. The expression and sub-cellular location of hnRNP K in HCC cell line of different cell densities were determined by immunocytochemical staining and Western blotting assay. Results.. Expression of hnRNP K was mainly detected in the nucleus of HCC cells. Twenty-one of the 30 hepatic cancer tissues had higher hnRNP K expression than the paired paracancerous tissues. Sixteen of the 20 HCC samples with HBV infection had an increased expression of hnRNP K; in the 10 HCC samples without HBV infection, 5 had an increased expression of hnRNP K. Immunocytochemical staining and Western blotting assay both showed that the expression of hnRNP K was increased in the nucleus of HCC cells with the increase of cell densities （30%, 50% and 90% confluence） ; the result of Western blotting assay also showed that there was no obvious change in hnRNP K expression in the cytoplasm of HCC cells of different cell densities（30%, 50%, 70% and 90% confluence）. Conclusion： The expression of hnRNP K is elevated in HCC tissues and has an increasing tendency in the nucleus with the increase of cell density. Infection of HBV may be associated with the up-regu- lation of hnRNP K in HCC cells.