发形霞水母毒素分离产物溶血活性的比较及其影响因素分析

Comparison of haemolytic activities of venom separations from jellyfish Cyanea capillata and their influencing factors

目的:分离发形霞水母刺丝囊毒素(nematocyst venom,NV)与无刺丝囊触手提取物(tentacle-only extract,TOE),比较二者的溶血活性,分析其影响因素。方法:采用自溶、离心方法分离发形霞水母NV和TOE,比较二者的溶血活性,观察提取物浓度、温度和pH值等因素对二者溶血活性的影响。结果:成功分离到NV与TOE;二者浓度相关溶血活性曲线均呈"S"形,溶血分数50%时所对应的质量浓度(HU50)分别为8μg/ml和67μg/ml,前者溶血活性强度约为后者的8.4倍;温度对二者溶血活性影响较大,最大溶血活性都出现在40℃;pH值对二者溶血活性也有影响,在pH8处二者都具有最大的溶血活性,但后者较前者更为敏感。结论:发形霞水母毒素分离产物NV和TOE均具有溶血活性,且前者强于后者;二者溶血活性受浓度、温度和pH值的影响。

Objective.. To separate the nematocyst venom （NV） and tentacle-only extract （TOE） from the jellyfish Cyanea capillata and to analyze the difference between their haemolytic activities and the influencing factors. Methods.. NV and TOE were separated by autolysis and centrifugation. The influence of concentration, temperature and pH value on the haemolytic activities of NV and TOE were observed. Results.. NV and TOE were successfully separated. The concentration-associated haemolytic curves were “S” shaped for both NV and TOE. The HU50 of NV and TOE were 8μg/ml and 67μg/ml, respectively; and the haemolysis of NV was about 8.4 times of that of TOE. Temperature had great influence on haemolysis of both and the highest haemolysis were both at 40℃. pH value also had influence on haemolysis. The strongest haemolysis activity was found both at pH 8 and TOE was more sensitive. Conclusion： NV and TOE both have haemolysis activity,with the activity of NV stronger than that of the latter. The haemolysis activity is influenced by concentration, temperature and pH value.