PARP抑制剂5-AIQ对CT26细胞侵袭及转移的影响

Effect of poly(ADP-ribose)polymerase inhibitor 5-AIQ on invasion and metastasis of CT26 cells

目的探讨多聚(腺苷二磷酸核糖)聚合酶[Poly(ADP-ribose)polymerases,PARPs]抑制剂5-氨基异喹啉酮[5-Aminoisoquinolinone.HCl,5-AIQ]对小鼠结肠腺癌CT26细胞基质黏附、运动、侵袭能力的影响。方法应用Westernblot检测5-AIQ对PARP表达的影响,细胞-基质黏附实验、细胞运动及侵袭实验观察5-AIQ抑制CT26细胞PARP后,其基质黏附、运动及侵袭能力的变化。结果5-AIQ处理组PARP表达明显弱于未处理组(P<0.05);黏附实验结果显示,与5-AIQ未处理组比较,5-AIQ100、500μmol/L处理组的吸光度值明显降低(P<0.01),其黏附抑制率分别是14.54%、21.69%;运动及侵袭实验中,5-AIQ500μmol/L处理组与未处理组的细胞数差别显著(P<0.05),其运动及侵袭抑制率分别是30.09%、37.47%。结论5-AIQ可抑制CT26细胞PARP的表达,并可降低CT26细胞基质黏附、运动及侵袭能力,5-AIQ在抗肿瘤侵袭转移中可能发挥作用。

Objective To study the effect of poly（ADP-ribose） polymerase （PARP） inhibitor 5-AIQ on the adhesion, migration and invasion of mouse colon adenocarcinoma cell line （CT26）. Methods The expres- sion of PARP after 5-AIQ treatment was detected Western blot. The cell adhesion, migration and invasion of CT26 after 5-AIQ treatment were observed by cell-matrix adhesion assay, cell migration assay and matrigel invasion assay. Results The expression of PARP in 5-AIQ-treated CT26 was weaker than that in 5-AIQ-untreated cells （P 〈 0.05）. In the adhesion assay, the OD value of 5-AIQ-treated cells was reduced as compared with untreated cells （P 〈0.05） and the inhibition rate of 100 μmol/L 5-AIQ treated cells and 500 μmol/L 5-AIQ treated cells was 14.54% and 21.69% respectively. Moreover, migration and invasion potential of CT26 were also reduced by 500 μmol/L 5-AIQ treatment （P 〈0.05）, and the inhibition rate was 30.09% and 37.47% for migration and invasion respectively. Conclusion PARP inhibitor 5-AIQ can reduce the expression of PARP, adhesion, migration and invasion of CT26 ceils, suggesting 5-AIQ probably plays a role against tumor metastasis.