不同基质、生长调节剂、插穗规格和代谢调节剂对‘蓝星’扦插生根的影响

Effects of different substrates,growth regulators,grades of cuttings and metabolism regulator on cutting propagation of Juniperus squamata ‘Blue Star’

该文研究了扦插基质、生长调节剂、插穗规格以及代谢调节剂处理对‘蓝星’扦插生根率和根系质量的影响,并分析了5种规格插穗的生根进程和生根型。结果表明:以珍珠岩为基质能显著提高生根率。3种生长调节剂中以ABT1#处理生根效果最好,对嫩枝和硬枝的生根率、生根量、偏根率影响显著。嫩枝插穗以ABT1#1000mg/L(速蘸2～3s)处理为最优组合,生根率为66.7%;硬枝插穗以ABT1#500mg/L(浸泡1h)处理效果最好,生根率为60.17%,偏根率仅28.0%。5种不同规格插穗生根效果明显不同,规格1插穗生根效果最好,生根率最高为80%;规格4次之,规格5生根效果最差。不同浓度代谢调节剂对3种规格插穗生根影响不同,其中1/4MS效果最好。规格5插穗经1/4MS+NaHSO3(200mg/L)处理后,与对照相比,生根率提高34.9%。5种不同规格插穗生根进程基本一致,但生根型明显不同,规格1～4插穗以皮部生根型为主,规格5插穗属于愈伤生根型。

Effects of substrates, growth regulators, grades of cuttings and metabolism regulators on the cutting propagation of Juniperus squamata ‘ Blue Star＇ were researched, and five different grades of cuttings were compared between rooting process and rooting type. Results showed that the rooting rate of the cuttings with the substrate of perlite increased significantly. ABT^1# was superior to other growth regulators on the rooting rate, rooting quality and one-side rooting rate of both softwood and wood cuttings. The optimized combinations were the softwood cuttings treated by perlite ＋ ABT^1# 1 000 mg/L（quickly dipped for 2-3 seconds）, and wood cuttings treated by perlite ＋ ABT^1# 500 mg/L （soaked for 1 hour）. The rooting rate reached 66.7 % and 60.17% separately,and for wood cuttings, the one-side rooting rate was only 28.0%. The rooting ability of cutting grade Ⅰ was the best with 80% rooting rate and better rooting qualities compared with others, the lower rooting ability followed was grade Ⅳ, Ⅱ, Ⅲ, and that of grade Ⅴ was the worst. Metabolism regulators （MS ＋ NaHSO3 ） had obvious effects on the rooting ability. The rooting rate of grade Ⅴ rose by 34.9% compared with the control, when treated by 1/4MS ＋ NaHSO3 （200 mg/L）.The same rooting process and different rooting characteristics of five grades of cuttings were found. The adventitious roots of cuttings from grade Ⅰ to Ⅳ mostly originated from cortex of cuttings, but grade Ⅴ from callus tissue of the cuttings＇ bottom section.