VEGF及KDR在骨肉瘤中的表达及其临床意义

Clinical Significance and Expression of VEGF and Its Receptor KDR on Osteosarcoma

目的:探讨血管内皮细胞生长因子(VEGF)与血管内皮细胞生长因子受体(KDR)结合,在骨肿瘤中的表达及其临床意义。方法:采用免疫组化染色方法检测105例骨肿瘤组织的VEGF与KDR的表达,并对其中85例骨肉瘤主要临床资料、病理分级及临床相关参数进行比较,用χ2检验进行统计学处理。结果:骨肉瘤中VEGF与KDR阳性表达率明显高于骨软骨瘤阳性表达率(P<0.0001)。VEGF与KDR阳性表达率在骨肉瘤临床分期中Ⅱb期、Ⅲ期明显高于Ⅰ期、Ⅱa期(P<0.001),并随着病理分级恶性程度增高而显著增加(P<0.01)。且在软组织浸润和转移组中VEGF与KDR阳性表达率明显高于非浸润和非转移组(P<0.005)。结论:VEGF与KDR在骨肉瘤中不同程度异常表达与肿瘤的临床分期、病理分级和浸润、转移呈正相关,提示VEGF与KDR的高表达可能与骨肉瘤的发生发展有关,尤其与骨肉瘤的浸润转移密切相关。

Objective： To study the clinical significance and expression of vascular endothelial growth factor （VEGF） and its receptor KDR on osteosarcoma. Methods：Using Immunohistochemical assay to detect the expression of VEGF and KDR in bone tumors from 105 patients, among which 85 cases were diagnosed as osteosarcoma, their clinical data, pathological grading and clinical related parameters were compared and processed statistically by chi square test. Results：The positive expression ratios of VEGF and KDR in osteogenic sarcoma were obviously higher than that in osteoenchondroma （ P 〈 0. 0001 ） , and higher in clinical stage Ⅱb and Ⅲ than in stage Ⅰ and Ⅱa （ P 〈 0. 001 ） , and markedly increased along with malignant degree based on pathological classification （ P 〈 0.01 ）. The positive ratios in those tumors with soft tissue infiltrating and metastasis were significant higher than that in the others （ P 〈 0. 005 ）. Conclusions： Abnormal expression of VEGF and KDR proteins in osteosarcoma were positively correlated with clinical stage, pathological grade and invasion and metastasis. It indicates that high expression of VEGF and KDR proteins may be correlated with the occurrence and development of osteosarcoma, especially with infiltrating and metastasis of osteosarcoma.