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豚鼠肌注庆大霉素10天和17天畸变产物耳声发射特性研究

Research on DPOAE of Guinea Pigs Treated with Gentamicin
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摘要 研究豚鼠肌注庆大霉素(GM)10和17 d耳声发射DP-gram和I/O曲线的特征性改变。选用健康豚鼠40只,随机分为4组:I组肌注GM 10 d,II组肌注等容量生理盐水10 d,III组肌注GM 17 d,IV组肌注等容量生理盐水17 d;采用CELESTA 503型耳声发射测试分析系统,观测四组豚鼠DP-gram和I/O曲线特征性改变,计数耳蜗外毛细胞缺失率。(1)I组DP-gram在4、6和8 kHz处DPOAE(Distortion product otoacoustic emission)幅值明显下降,同II组相比有显著性差异(t为2.52、1.92、2.10,P<0.05)。III组DP-gram在3、4、6和8 kHz处明显下降,同IV组相比有显著性差异(t为3.27、2.81、2.92、3.13,P<0.01)。I、III组I/O曲线在不同频率不同刺激强度下DPOAE幅值明显下降。(2)刺激强度L1<60 dB SPL,I、III组I/O曲线在4、6和8 kHz引起DPOAE反应幅值为0 dB时最小刺激强度较相应对照组大。(3)II、II组在8 kHz阈移均数分别为5.0±3.8 dB和25±6.2 dB。(4)I、III组外毛细胞缺失率分别为22.16%和48.36%,同相应对照组相比有显著性差异(P<0.01)。豚鼠肌注庆大霉素10 d和17 d,畸变产物耳声发射频率特异性好,DPOAE的改变与耳蜗形态学变化一致,DPOAE可以作为监测庆大霉素耳毒性敏感指标。 This study was designed to disclose the specific changes of distortion product otoacoustic emission (DPOAE), including DP-gram and I/O -gram in guinea pigs treated with GM for 10 and 17 days. Forty guinea pigs were divided into four groups:groupⅠ (treated with GM for 10 days), group Ⅱ (treated with normal saline for 10 days) ,group Ⅲ (treated with GM for 17 days), group Ⅳ (treated with normal saline for 17 days). DPOAE including DP-gram and DP-I/O was recorded by use of CELESTA 503 Cochlear Emission Analysis and the out hair cell loss was numerated. ( 1 ) The amplitude in group Ⅰ showed significant reduction at frequencies of 4, 6, 8 kHz. There were significant differences, oompared to group II (t = 2.52,1.92,2.10, P 〈 0.05). The amplitude in group Ⅲ also decreased at frequencies of 3, 4, 6, 8 kHz. There were also significant differences omapared to group Ⅳ ( t = 3.27,2.81,2.92,3.13, P 〈 0.01 ). The amplitude of DP-I/O in group Ⅰ and group Ⅲ at different frequencies and stimulus levels showed reduction. (2)At the level L1 〈60 dB SPL, the stimulus levels to elicit a 0 dB response in group Ⅰ and group Ⅲ were higher than these in corresponding control groups, respectively. (3( The mean of threshold shift was 5.0±3.8 dB in group Ⅰ and 25.0±6 dB in group Ⅲ at 8 kHz frequency. (4)The out hair cell loss was 22.16% in group Ⅰ and 48.36% in group Ⅲ,both showed significant difference as compared to controls,respectively (P〈0.01). The employment of DPOAE can be a useful tool in monitoring ototoxicity of GM for its sensitivity and specificity in guinea pigs treated with GM. The changes in DPOAE were consistent with these in histology.
出处 《生物医学工程学杂志》 CAS CSCD 北大核心 2008年第1期57-60,共4页 Journal of Biomedical Engineering
关键词 豚鼠 畸变产物耳声发射 庆大霉素 Guinea pig Distortion product otoacoustic emission (DPOAE) C-entamicin(GM)
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