姜黄素／聚乳酸-乙醇酸共聚物复合薄膜的制备及抗凝血研究

Preparation and Anticoagulation of Curcumin/Poly(lactic acidco-glycol acid) Composite Films

血管支架内再狭窄是血管支架临床应用中最突出的问题,药物洗脱支架的问世成为冠心病介入治疗的一个重要里程碑。但是目前的药物洗脱支架还存在抗凝血不足的问题,药物洗脱支架植入晚期血栓形成的病例在临床上有所报道。姜黄素具有抗增生以及抗凝血等多种药理活性,有望成为药物洗脱支架的新颖药物。我们以可降解高分子材料聚乳酸-乙醇酸共聚物(PLGA)为载体分别制备了三种浓度(3wt%、5wt%、8wt%)的姜黄素复合薄膜。采用傅立叶变换红外光谱研究了复合薄膜的组成成分,结果显示:姜黄素与PLGA的特征峰在复合薄膜的红外图谱中均有出现;体外血小板黏附实验结果显示姜黄素复合薄膜表面的血小板黏附数量减少,较少团聚、变形和激活;复合薄膜的部分凝血活酶时间(APTT)长于纯PLGA薄膜的APTT,这都表明姜黄素/聚乳酸-乙醇酸共聚物复合薄膜的抗凝血性能得到改善,且复合薄膜的抗凝血性能在实验药物浓度范围内随着药物含量的增加,材料的抗凝血性能进一步提高。

In-stent restenosis is the major problem in clinical application of coronary stent. Drug-eluting stent became a landmark in the treatment of coronary disease. However, thrombosis is still a problem of drng-eluting stent. There has been clinical report indicating that thrombosis sometimes is induced by drug-eluting stent implantation in late stage. Curcumin could be used for drug-eluting stent due to its antithrombogenity and antiproliferative properties. In this paper, three weight percent curcurnin-loaded films （3wt%, 5wt%, 8wt%） were prepared using a biodegradable polymer （poly {lactic acid-co-glycol acid）, PLGA） as the carrier of curcumin. The component of curcumin-loaded film was analyzed by Fourier transform infrared spectroscopy （FTIR）, and the major peaks of curcumin and PLGA were both observed in the composite film. The result of in vitro platelet adhesion test shows that the number of adhered platelet reduces, and few aggregated and activated plate.lets are oiled. For all composite films, activated partial thromboplastin time （APTT） increases. The results indicate that the curcumin-loaded films have better anticoagulative effect when compared with PLGA. In addition, all anticoagulation tests indicate ＂the higher the drug content in the film, the better the anticoagulative effect＂.