新辅助化疗对大肠癌细胞凋亡和P53表达的影响

Affection of Short-Term Neoadjuvant Chemotherapy on Cell Apoptosis and Expression of P53

目的:研究新辅助化疗能否引起大肠癌细胞的凋亡和对大肠癌组织P53表达的影响。方法:选取中南大学湘雅二医院老年外科2004年9月至2006年9月收治的大肠癌手术病人68例,分别采用脱氧核糖核苷酸末端转移酶介导的缺口末端标记法(term inal-deoxynucleotidyl transferase med iatednick end labeling,TUNEL)和免疫组化S-P法对68例患者大肠癌组织中的肿瘤细胞的凋亡指数(Apoptotis index,AI)和P53表达进行检测。结果:新辅助化疗组肿瘤细胞的AI均数为3.56%,明显高于对照组的2.29%(P<0.01)。新辅助化疗组P53阳性表达率为22.60%,明显低于对照组的33.60%(P<0.05)。结论:新辅助化疗可以显著诱导大肠癌细胞凋亡,降低凋亡相关基因P53的表达。

Objective： To investigate whether neoadjuvant chemotherapy could induce tumor cell apoptosis and review its effect on expression of P53 in carcinoma of large intestine. Methods： Apoptosis index of tumor cells was assayed by terminal -deoxynucleotidyl transferase mediated nick end labeling（TUNEL） and positive rate of P53 expression was examined by immunohistochemical S - P in 68 carcinoma of large intestine samples. Results：Tumor cell AI in neoadjuvant group（3.56% ）was significantly higher than that in control group（2. 29% ） （P 〈 0. 01 ）. P53 positive expression rate in neoadjuvant chemotherapy group （ 11/38, 28.9% ） was significantly lower than that in control group （17/30, 56.7% ） （P 〈 0. 05 ）. Conclusion： Neoadjuvant chemotherapy could induce cell apoptosis and degrade the positive rate of P53 expression in carcinoma of large intestine.