胆红素诱导海马区神经细胞凋亡实验研究

Experimental Research of Bilirubin Inducing Apoptosis of Nerve Cells in Hippocampus Region

目的:从分子水平探讨胆红素对神经细胞的毒性机理,为新生儿胆红素脑病的防治提供新的途径。方法:制作高胆红素血症动物模型,观察海马区神经细胞的组织学改变,检测脑组织胆红素浓度及海马区神经细胞凋亡率,并探讨其间相关性。结果:高胆红素血症时,海马区神经细胞出现凋亡的组织学改变,神经细胞凋亡率明显增高,与脑组织胆红素浓度呈正相关。结论:胆红素通过诱导海马区神经细胞凋亡介导其神经毒性的产生。

Objective： To explore the toxicity mechanism of bilirubin that effects nerve cells from the molecular level of bilirubin, To provide a new way of prevention and treat ment of the neonatal bilirubin encephalopathy. Methods： To make hyperbilirubinemia animal model. To observe the histological changes of nerve cells in hippocampus region , To detecte the bilirubin concentration in the brain tissue and the apoptosis rate of nerve cells in hippocampus region and explore thief relativity. Results：The nerve cells in hippocampus region appeared the histological changes of apoptosis in the state of hyperbilirubinemia; and the apoptosis rate of nerve ceils was significantly increased. There was positive relationship with the bilirubin concentration of brain tissue. Conclusion： Bilirubin neurotoxicity can be formed by inducing the apoptosis of nerve cells in hippocampus region.