三七对酒精性肝病大鼠肝组织学和TNF-α影响

Effects of Sanchi on Hepatic Expression of TNF-α in Alcoholic Hepatopathy Rats

目的:观察三七对酒精性肝病大鼠肝组织学和TNF-α影响。方法:70只SD雄性大鼠随机分为正常组10只,模型组、三七高、低剂量组和硫普罗宁组各15只,连续14周建立酒精性肝病模型。三七高剂量组、低剂量组在造模的同时,每天下午分别灌服1.2g/kg体重和0.6g/kg体重的三七粉,硫普罗宁组在造模的同时每天下午灌服0.1g/kg体重的硫普罗宁,均连续14周。ELISA法检测肿瘤坏死因子(TNF-α)。常规HE及M asson染色,光镜观察肝组织的脂肪变、炎症及纤维化程度。结果:模型组大鼠肝组织脂肪变及炎症程度计分、血清TNF-α水平较正常组明显增高(P<0.01)。三七高、低剂量组,硫普罗宁组大鼠肝组织脂肪变及炎症程度、血清TNF-α水平较模型组明显减轻(P<0.01,P<0.05)。结论:(1)用白酒-玉米油-吡唑混合液灌服大鼠14周能成功复制ALD模型。(2)三七可明显减轻酒精性肝病大鼠肝组织脂肪变和炎症程度。⑶三七能显著降低血清TNF-α水平,这可能是其有效防治酒精性肝病的发生发展的重要机制之一。

Objective ： To characterize the effects of Sanchi on the expression of TNF -α in hepatic tissues of alcoholic hepatopathy rats. Methods： 70 SD male rats were randomly divided into five groups： normal group （ n = 10） , control group （n = 15） , high -dose Sanchi group （ n = 15）, low -dose Sanchi group （n = 15） , and Tiopronin group （ n = 15）. All rats were induced alcoholic hepatopathy in 14 weeks. Rats in high -dose Sanchi, low dose Sanchi, and Tiopronin groups, were treated every afternoon during the 14 - week induction of hepatopathy, with 1.2g/kg Sanchi, 0.6g/kg, and 100mg/kg Tiopronin, respectively. TNF -α were determined by ELISA. Histopathologlcal changes of hepatic tissues （i.e. steatosiss, inflammation, and fibrosis） were assessed by microscopic examination of HE and Masson staining of the right lobe of livers. Results： Compared with rats from the normal group （treated with ddH20）, rats in the control group have significantly higher steatosis, inflammation, liver fibrosis, and increased level of TNF -α（ P 〈0.01 ）. More importantly, treatment with high -dose, low- dose Sanchi, or Tiopronin resulted in less severe steatosis, inflammation, fibrosis in the liver, and reduced the levels of TNF -α （ P 〈 0. 01, P 〈0.05）. Conclusion： （ 1 ） Daily dosing of alcohol, corn oil, and pyrozole for 14 weeks faithfully replicated the alcoholic hepatopathy symptoms observed in the ALD model, such as hepatic steatosis, immune cell infiltration, blood -fat disturbance, increase in serum AST/ALT activity, and liver fibrosis. （2） Sanchi can significantly alleviate the symptoms in the alcoholic hepatopathy rats including steatosis, immune cell infiltration, reduce serum AST/ALT activity and liver fibrosis. （3） Sanchi can markedly reduce the levels of TNF -α in senun. This may serve as one of the critical mechanisms through which Sanchi efficiently prevents alco- holic liver diseases.