槟榔次碱对未孕大鼠离体子宫平滑肌运动的影响

Study on Effect of Arecaidine on the Isolated Uterus of Rats

目的:观察不同浓度的槟榔次碱对未孕大鼠离体子宫平滑肌运动的影响及研究其作用机理。方法:取大鼠卵巢及子宫叉处的子宫角,沿系膜纵轴剖开,置于恒温的平滑肌槽中,连接生理记录仪,观察槟榔次碱对子宫平滑肌运动的影响,以及分别加入五种阻断剂后.观察槟榔次碱对子宫作用的影响。结果:不同剂量的槟榔次碱能显著增强子宫平滑肌收缩的频率、振幅,增大收缩面积并呈正相剂量效应关系,而对收缩波的持续时间无明显影响,阻断M-受体后,槟榔次碱的增强作用即消失。而H1受体、L-型钙通道、α-受体、前列腺素合成酶分别被阻断或抑制后,槟榔次碱的增强作用未受到明显影响。结论:槟榔次碱能显著增强离体子宫平滑肌的收缩作用,并且主要是通过兴奋M-受体而发挥作用。

Objective ： To investigate the effects of arecaidine on contractile activity of isolated uterine smooth muscle strips of virginal rats and explore their action mechanism. Methods ： Using unpregnant female wistar rats uterine smooth muscle strips that were cut along the Iongitudinal axis of uterus to observe the effects of arecaidine on contractile activity of isolated uterine smooth muscle strips of virginal rats. Each strip was suspended in a constant temperature smooth muscle groove and connected to an isometric force transducer and the contractile response was recorded simultaneously by physiological recorder. Five antagonists were lnjected to study arecaidine mechanisms respectively. Results ：Different dosages of arecaidine could significantly increase the mean contractile amplitude and the frequency of uterus, and enlarge the spike area of uterine contraction. When M receptor was blocked, the effects of arecaldine disappeared. When a receptor and H1 receptor and L- Voltage- dependant calcium channels and prostaglandin synthesized were blocked, the effects of arecaidine had no evidently changed. Conclusion ：Arecaidine can effectively enhances the contractile activity of uterine smooth muscle strips of unpregnancy rats which may result from exciting the M receptor.