大孔树脂分离纯化紫苏总黄酮的工艺研究

Study on Extraction of Flavones of Perilla Frutescens with Macroporous Resin

目的:筛选分离纯化紫苏总黄酮的最佳树脂,并确定其工艺条件。方法:以总黄酮吸附量和回收率为考察指标,采用静态吸附分离法确定适合的大孔吸附树脂;采用动态吸附分离法确定分离条件。结果:AB-8树脂对紫苏总黄酮有良好的吸附分离性能。其动态分离纯化工艺条件为:紫苏总黄酮上样液浓度为1.5mg/mL,最大吸附量为11.5mg/g,吸附速度为1.0mL/m in,95%乙醇2倍柱体积1.0mL/m in洗脱,树脂可重复使用3次。结论:该方法操作简便、有效,适于紫苏总黄酮的吸附纯化。

Objective： To study the technological conditions and parameters of the purification process of total flavones in perillafrutescens （L.）Britt. with macroreticular resin. Methods： The total flavones adsorption capacity and recovery rate were used as the evaluation criteria. The static adsorption and separation were used in investigation of macroporous adsorption resin; the dynamic adsorption and separation were used in studying the condition of adsorption and separation. Results： The properties of AB -8 resin is good for absorption and desorption of total flavones in the following technological condition ： the concentration of the extract was 1.5mg/mL; the maximum adsorbing capacity for total flavones 11.5mg/g; the current velocity was 1.0mL/min; the eluting reagent was 95% ethanol （twice as the volume of the resin） ;the eluting velocity was 1.0mL/min ; and the AB- 8 resin could be used three times, repeatedly. Conclusion： It is simple and efficient to separate the total flavones of perilla frutescens under the technological condition.