枸杞髓部细胞悬浮培养及胚状体发生

SOMATIC EMBRYOGENESIS AND PLANT REGENERATION FROM LYCIUM BARBARUM L.SUSPENSION PITH CELL

应用枸杞髓部组织进行离体培养，建立细胞系，诱导胚状体发生。结果在合不同激素的4种MS培养基上都访导出了愈伤组织，诱导频率在56.7％－95．7％，其中以MS＋6BA0.1mg／L＋NAA0.5mg／L＋蔗糖3％培养基诱导出的愈伤组织颗粒小，分散性能好，此愈伤组织在2－3次继代培养后，转入液体培养基中进行振荡培养，24h后获得大量单细胞，经过多次继代培养，建立稳定的单细胞悬浮系。悬浮细胞在液体培养基中，培养8－10d可获得大量胚状体。味集胚状体转移到MS＋6BA0.2mg／L的固体培养基上，胚状体能够萌发并形成大量绿色小芽，小芽转入生根培养基中20d得到完整植株。

Calli were induced on 4 MS medium with different hormone content, the induction frequency was 56. 7 - 95. 7%. The calli induced on medium MS+ 6BA 0.1mg/L+su-crose 3% were the smallest in particle size and had good dispersion ability- After 2-3 successive transter culture with the calli, a shake suspending culture was made in liquid medium and a large quantity of single cella was obtained after 24 hours. With another several times of successive transfer culture, a stable cell st1spending culture line was established. Afrer 8 - 10days, a large quantity of emblyoids were obtained in the liquid medium. The emblyoids were then moved onto solid medium and green buds appeared. After culturing the buds on rooting medium for 20 days, complete plants were obtained.