凋亡相关蛋白Survivin及hTERT mRNA在肾细胞癌中的表达及意义

Expression and significance of Survivin protein and telomerase hTERT mRNA in renal cell carcinoma

目的研究凋亡抑制蛋白Survivin和hTERT mRNA在肾细胞癌组织中的表达及意义。方法采用免疫组化和原位杂交法检测46例肾细胞癌组织和15例对照组织(癌旁正常肾组织)中Survivin蛋白和hTERT mRNA的表达。结果46例肾细胞癌组织中Survivin的阳性表达39例,阳性表达率为84.78%,而15例正常组织中Survivin蛋白仅1例阳性表达,阳性率为6.67%,肾细胞癌组织Survivin蛋白的阳性表达率与正常比较差异有显著性(P<0.01);46例肾细胞癌组织中hTERT mRNA阳性表达41例,阳性率为89.13%,15例正常组织中hTERT mRNA阳性表达仅有1例,阳性率为6.67%,两组hTERT mRNA阳性表达率差异有显著性(P<0.01)。结论肾细胞癌组织中存在Survivin蛋白和hTERT mRNA过表达,这可能与肾细胞癌的发生发展有关。

[Objective] To investigate the expression and significance of Survivin protein and telomerase hTERT mRNA in renal cell carcinoma. [Methods] The expression of Survivin protein and telomerase hTERT mRNA were detected respectively with immunohistochemistry and in situ hybridization in 46 cases of renal cell carcinoma and 15 cases normal controls. [Results] Survivin protein positive expression was found in 39 of 46 renal cell carcinomas （84.78%） and 1 case positive expression in are of the normal samples （6.67%）, there was significant difference between the renal cell carcinoma and normal samples（P 〈0.01）. hTERT mRNA positive expression was found in 41 of 46 renal cell carcinoma （89.13%） and in 1 of 15 normal samples （6.67%）, there was significant difference between the renal cell carcinoma and normal samples （P 〈0.01）. [Conclusions] Overexpression of Survivin protein and telomerase hTERT mRNA in renal cell carcinoma may play an important role in carcina genesis and development of renal cell carcinoma.