3-吲哚甲醇的抗肿瘤作用

Experimental study on antitumor activity of indole-3-carbinol

目的探讨硫代葡萄糖苷的降解产物3-吲哚甲醇(IC)在体内、外的抗癌活性,为十字花科蔬菜的防癌作用以及从蔬菜中筛选药用成分提供理论依据。方法体内试验以荷瘤小鼠实体瘤重、抑瘤率及对脾脏、胸腺指数的影响为指标,评价IC对S180移植性肉瘤的抑制作用;长期实验在饮水中加入化学致癌剂甲硝基亚硝基胍(MNNG),诱发小鼠前胃鳞癌及癌前病变,同时给予不同剂量IC进行预防,观察癌前病变和癌变发生率;体外采用MTT法,观察IC对HeLa细胞增殖的抑制作用。结果IC在体内能明显抑制实体瘤重,使荷瘤小鼠的脾脏和胸腺指数下降,表现出一定的免疫抑制作用;长期试验能明显降低MNNG诱发的癌前病变和癌变发生率;在体外对HeLa细胞有明显的生长抑制作用,且作用随药物浓度增加而加大。结论IC具有一定的抗肿瘤活性。

OBJECTIVE To investigate the antitumor activity of Indole - 3 - carbinol （ IC）, the hydrolytic products of glucosinolates, in vivo and in vitro, and to screen medicine materials from diet vegetables and provide theory support for the anticarcinogenicity of cruciferus. METHODS The inhibition effect of IC to S180 cells in vivo was evaluated by the tumor weight of the mice bearing tumor and the tumor inhibition rate. The effects on spleen and thymus were also observed. Long term carcinogenesis model was induced by N- methyl -N＇-nitro- N- nitrosoguanidine （MNNG）. The in vitro anticancer effect was studied by MTF assay. RESULTS It was obtained that HeLa cells were obviously inhibited by the influence of IC in vitro. And there was positive correlation between the inhibitory effect and IC concentration. Furthermore, the IC also had the anticancer activity to the S180 cells of the mice bearing tumor. The declining of spleen and thymus index indicated that IC had the effect of immunosuppressant. IC can reduce the precancerous affections and cancer ratio in long term carcinogenesis experiments. CONCLUSION IC has some effect on inhibition of tumor.