光源对金丝桃素光动力体外抗肿瘤作用的影响

Effect of light wavelength on photodynamic therapy of hypericin and extract from Hypericum performatum L. on HepG2 cancer cell line of human liver in vitro

目的研究金丝桃素和贯叶金丝桃中金丝桃素提取物对人肝癌细胞株HepG2的体外杀伤效应及光源对其抗肿瘤作用的影响。方法分析金丝桃素及金丝桃素提取物的吸收光谱,用不同波长的光源激发进行体外细胞毒试验,采用显微镜观察、MTT法分析和DNA电泳等方法评价金丝桃素及金丝桃素提取物的抗肿瘤效果。结果590 nm的黄光激发金丝桃素和金丝桃素提取物对HepG2细胞有显著的体外杀伤效应,生长抑制率分别为51.5%、83.1%,其他波长光源的作用不显著。结论金丝桃素的光动活性依赖于光源的波长,受适当光源激发的金丝桃素及金丝桃素提取物能显著抑制HepG2细胞的生长,显示金丝桃素有良好的光动力治疗肝癌的开发前景。

OBJECTIVE To investigate the antitumoral effect in vitro and light wavelength effect of hypeficin and extract from Hypericurn performaturn L. on HepG2 cancer cell line of human liver. METHODS To detect the absorption spectra of hypeficin and extract, use light irradiation of different wavelength for photocytotoxicity test, and assess antitumor effect of hypericin and extract by microscopy, MTT assay and DNA ladder gel electrophoresis. RESULTS The irradiation of 590 nm （yellow） to hypeficin and extract had obvious effect on killing HepG2 cell in vitro. The rates of growth inhibition were 51.5 and 83.1%. CONCLUSION Our study demonstrates that the photodynamic activity of hypericin and extract depends on light wavelength. Hypericin and extract can obviously decrease the growth of HepG2 cancer cell. Hypeficin is a powerful naturally photosensitizer and should have a good prospect for further investigation.