摘要
目的测定郁金类药材中的吉马酮、莪术二酮。方法采用HPLC法,色谱柱为C18柱,提取溶剂为甲醇,流动相为乙腈-水(75-25),流速0.8ml·min^-1,检测波长244nm。结果加样回收率95%~105%,RSD分别为1.34%、1.87%(n=5)。结论所建方法简便、准确,为郁金类中药材吉马酮、莪术二酮的测定提供了参考依据。
OBJECTIVE To determine the content of germacrone and curdione in Radix Curcumae by HPLC. METHODS C18 kromasil was the chromatographic column, the mobile phase was acetonitrile and water(75 : 25 )with a flow rate of 0. 8 ml·min^-1, the detection wavelength was set at 244 nm. RESULTS The recovery of germacrone and curdione was 95% - 105%. RSD was 1.34%, 1.87% ( n = 5 ) respectively. CONCLUSION The method is simple and reliable and it can be used for the content determination and the quality control of Radix Curcumae.
出处
《华西药学杂志》
CAS
CSCD
北大核心
2008年第1期105-106,共2页
West China Journal of Pharmaceutical Sciences
基金
四川省科技厅育种攻关项目郁金规范化研究(2006yzgg12-82)
成都市科技局项目郁金种质资源评价及其质量标准规范化研究(06GGYB084SF-034)