HPLC测定安神补心丸中的五味子醇甲、五味子酯甲、五味子甲素、五味子乙素和丹参酮Ⅱ_A

Determination of schizandrol,schisantherin,deoxyschizandrin,γ-schisandrin and tanshinoneⅡ_A in Anshen Buxin pills by HPLC

目的测定安神补心丸中的五味子醇甲、酯甲、甲素、乙素和丹参酮ⅡA。方法样品用超声提取，采用RP—HPLC法直接测定，用十八烷基键合相，乙腈-水-冰醋酸（60：40：0．1）为流动相，流速1ml·min^-1，检测波长220nm。结果五昧子醇甲、酯甲、甲素、乙素和丹参酮ⅡA分别在0．0006～0．1230μg（r=0．9999）、0．0013～0．2550μg（r=0．9998）、0．0017～0．3391μg（r=0．9997）、0．0026～0．5260μg（r=0．9999）、0．0020～0．4000μg（r=0．9999）与峰面积具有良好线性关系。平均加样回收率分别为97．3％、98．8％、101．1％、98．1％和100．2％。RSD（n=5）分别为3．1％、2．2％、3．7％、3．2％和2．0％。方法用于安神补心丸中五味子醇甲、酯甲、甲素、乙素和丹参酮ⅡA的含量测定，RSD（n=5）分别为1．1％～3．7％、1．7％～3．8％、2．8％-4．8％、3．1％～4．4％和1．8％～3．1％。结论所建方法灵敏度高，准确，简便快速。

OBJECTIVE To develop a method to determine the content of schizandrol, schisantherin,deoxyschizandrin,γ- schisandrin and tanshinone Ⅱ A in Anshen Buxin pills. METHODS The samples were extracted with methanol by ultrasonic wave and then filteration. The determination was performed by HPLC on Shim - pack VP - ODS C18 column using acetonitrile - water - acetic acid glacial（60 ： 40 ： 0. 1 ） as a mobile phase, the flow rate was 1.0 ml·min^-1,the detection wavelength was 220 nm. RESULTS The linear range of schizandrol, schisantherin, deoxyschizandrin, γ - schisandrin and tanshinone ⅡA were 0. 0006 - 0. 1230 μg （ r = 0. 9999 ）, 0. 0013 -0. 2550 μg （r = 0. 9998 ） ,0. 0017 -0. 3391 μg （r = 0. 9997 ）, 0. 0026 -0. 5260 μg （r = 0. 9999 ） and 0. 0020 - 0. 4000 μg （ r = 0. 9999 ）, respectively. The average recoveries of five components were 97.3% , 98.8% , 101. 1% , 98. 1% and 100. 2%. CONCLUSION The method is accurate,quick, sensitive.