RP-HPLC测定2种桃叶珊瑚属药用植物中的桃叶珊瑚苷

Determination of aucubin in two medicinal plants of Aucuba

目的测定桃叶珊瑚、峨眉桃叶珊瑚中的桃叶珊瑚苷。方法药材用70％乙醇回流提取，RP—HPLC法测定，色谱柱为Symmetry shield RP18柱（250mm×4．6mm，5μm）；流动相用甲醇-水（12：88）；检测波长203nm；柱温25℃；流速1．0ml·min^-1。结果桃叶珊瑚苷在203nm处有最大吸收，在0．72～2．88μg线性关系良好（r^2=0．9998），桃叶珊瑚、峨眉桃叶珊瑚中桃叶珊瑚苷的含量分别为4．31％、3．07％，平均回收率分别为98．94％、98．87％，RSD分别为2．04％、1．74％（n=6）。结论所建方法操作简便，重复性好，专属性强，准确可靠。

OBJECTIVE A method was established for determination of aucubin in Aucuba chinensis and Aucuba omeiensis. METHODS Aucubin was extracted by EtOH -H2O （70 ： 30） with reflux extraction and determined by RP- HPLC method, column： Symmetry shield RPls column （250 mm ×4.6 mm,5 μm）, mobile phase： MeOH- H2O（ 12 ： 88）, detection wavelength： 203 nm, column temperature ： 25 ℃, flow rate ： 1.0 ml. min - 1. RESULTS The maximum absorption wavelength of aucubin was 203 nm. The calibration curve was linear over the concentration range of 0.76 μg to 2.88 μg for aucubin contents. The aucubin contents in A. chinensis and A. omeiensis were 4.31% , 3.07% respectively. The average recoveries were 98.94% , 98.87% , RSD were 2.04% , 1.74% （n = 6）. CONCLUSION The method is proved to be repeatable, specific, accurate and easy to operate.