摘要
以玉米芯为主要原料,通过单因素和正交试验对绿色木霉HY-7液体发酵产纤维素酶的最佳工艺条件进行优化。结果表明,最佳产酶条件为:250mL三角瓶添加Mandels无机营养液75mL,添加玉米芯2.0%,蛋白胨0.05%,吐温800.1%,调整pH值为5.0;菌龄78h,接种量4.0%,转速150r/min,28℃恒温振荡培养102h,该条件下,其酶活为401.7u/mL,比优化前提高了44.6%。
Through single factor test and orthogonal experiments, the technical conditions of liquid fermentation of corn cob by trichoderma viride HY-07 to produce cellulase were optimized. The optimum conditions for cellulase yield were summed up as follows： 75 mL Mandels inorganic nutrition liquid was added in 250 mL flask with, then 2.0 % corn cob, 0.05 % peptone, and 0.1% Tween80 were added, then pH value adjusted to 5.0 with seed age as 78 h and 4.0 % inoculation quantity, then the rotation speed was at 150 r/min, and after 102 h culture at 28℃, the activity of the produced cellulase reached 401.7 u/mL, 44.6 % higher than that produced by unoptimizable techniques.
出处
《酿酒科技》
北大核心
2008年第2期30-33,共4页
Liquor-Making Science & Technology
基金
陕西省科技攻关项目(项目编号:2007JK01-13-2)
咸阳市科技攻关项目资助(项目编号:K0311-2)
陕西科技大学自然科学基金项目资助(项目编号:ZX06-17)
关键词
纤维素酶
绿色木霉
液体发酵
优化
cellulase
trichoderma viride
liquid fermentation
optimization
