里氏木霉纤维素酶在酒精生产中应用的研究

Studies on the Application of Cellulase of Trichoderma Reesi in Alcohol Production

本文探讨了里氏木霉纤维素酶的生产、性质及在酒精生产中的应用。采用固态培养法生产纤维素酶，最佳培养条件为：ｐＨ４．４～４．６，温度２８～３０℃，物料水份６８～７０％，培养４～５天，在此条件下，其ＣＭＣＮａ酶活可达９０００～１２０００ｍｇＧ／ｇ·ｈ。纤维素酶应用于以玉米为原料的酒精生产，其最适使用工艺为：添加量每克原料１２单位，１／３的纤维素酶在调浆时加入，２／３的纤维素酶在糖化后加入。在此试验条件下，玉米原料出酒率达３４．４１％，比对照提高１．４５％，相对出酒率提高４．４％

The persent article investigated the producing and performance and application in alcohol production of cellulase of trichoderma ressei.The cellulase producing was used with the method of solid-state culture.The optimum culture conditions of it were as follows:pH4.4～4.6,temperature 28～30℃,moisture content of culture medium 68～70%,and cultivation time4～5day.With such conditions,its enzyme activities of CMCNa reached 9000～12000mgG/g·h.The optimum reaction pH of cellulase was4.8,and the optimum reaction temperature of it was50℃,When cellulase was used in alcohol production,the alcohol rate of corn materials reached34.41%,it had increased by 1.45% over the compare.