宫颈上皮内瘤样病变和宫颈癌中CD44v6和P63蛋白表达及其意义

Expression and significance of CD44v6 and P63 protein in cervical intraepithclial neoplasia and cervical cercinoma

目的探讨CD44v6和p63蛋白在宫颈上皮内瘤样病变(C IN)和浸润宫颈鳞癌(ICC)中的表达及其临床意义。方法应用免疫组织化学方法,检测30例ICC、20例C IN和10例正常宫颈组织(NCE)中CD44v6和p63蛋白的表达水平,并结合临床病理特征进行分析。结果(1)在ICC组织中CD44v6蛋白表达阳性率为80.0%(24/30),明显高于C IN(35.0%,7/20,P<0.05)和NCE(30.0%,3/10,P<0.05)。(2)全部30例ICC和10例C IN中p63染色均为阳性。ICC分化程度越低,阳性细胞数越高、细胞的着色越深。NCE中仅基底层及上基层细胞阳性。(3)p63表达与ICC组织学分级和淋巴结转移相关(P<0.05)。结论CD44v6和p63可能参与了调控ICC的发生、发展过程,可作为ICC早期诊断指标,其高表达预示ICC预后不良。

Objective To study clinical significance of CD44v6 and p63 protein expression in cervical intraepithclial neoplasia（CIN） and invasive carcinoma of cervix （ICC）. Method Immunohistochemical method was used to examine the expression of CD44v6 and p63 protein in 30 cases of ICC,20 cases of CIN and 10 cases of normal cervical epithelium（NCE）. Results （ 1 ） The positive rates of CD44v6 expression of in ICC（ 80. 0% ,24/30） was significantly higher than that in CIN（35% ,7/20,P 〈0.05） and NCE （30. 0% ,3/10,P 〈0.05）. （2） p63 in all 30 cases of ICC and 20 cases of CIN had positive reaction. Expression of p63 was strongly associated with poor differentiation of ICC. （3） Expression of CD44v6 and p63 protein was correlated with tumor grade and lymph nodes metastasis in ICC （P 〈 0.05 ）. Conclusions CD44v6 and p63 protein may be closely correlated with tumor development. CD44v6 and p63 may be a early diagnostic index for ICC. High expression of CD44v6 and p63 protein indicate poor prognosis.