SPR生物传感器测定Aβ(1-40)与Cu(Ⅱ)结合的探索

Investigation on Aβ(1-40) and Cu(Ⅱ) association by SPR biosensor

采用表面等离子共振(SPR)生物传感器,分别固定Aβ及其单克隆抗体于传感芯片表面,实时监测Aβ与Cu(Ⅱ)混合前后与Aβ单抗体特异的结合过程,及Cu(Ⅱ)与Aβ亲和结合及解离情况.结果显示:Cu(Ⅱ)影响Aβ与Aβ单抗体的特异结合能力,加入Cu(Ⅱ)后Aβ不能与Aβ单抗体起特异免疫结合反应;Aβ与Cu(Ⅱ)的结合过程为快结合快解离的动力学过程.

Aβ/Aβ monoclonal antibody is coupled on CM5 chip. The association between Aft with/without copper and Aft monoclonal antibody is detected in situ, and that between Aft and copper is measured on the SPR sensorgram. The results show that Copper effects on the specific association between Aft and its monoclonal antibody. After adding Cu（Ⅱ） to Aft solution, Aft can not bond to its monoclonal antibody. The affintive process of Aft and copper is due to the rapid association and disassociation.