rhKD/APP var在慢性肝损伤中的保护作用

Study on protective effects of reorganization human Kunitz protease inhibitor domain of amyloid protein precursor variant on chronic liver in jury in Wistar rats

目的探讨重组人淀粉样蛋白前体Kunitz型蛋白酶抑制剂结构域变异体(reorganization human Kunitz protease inhibitor domain of amyloid protein precursor variant,rhKD/APPvar)对实验鼠慢性肝细胞损伤的保护作用。方法利用四氯化碳建立Wister大鼠慢性肝损伤实验动物模型,并给予rhKD/APPvar观察其对实验鼠血清丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、白蛋白(ALB)、白蛋白/球蛋白(A/G)、唾液酸(SA)、胆碱酯酶(CHE)、碱性磷酸酶(ALP)、肝组织羟脯氨酸(hydroxyproline,Hyd)含量以及肝组织的病理改变的影响。结果rhKD/APP明显降低血清ALT、AST、ALP,明显增高血清ALB、A/G、SA、CHE值。肝组织Hyd明显降低。鼠肝组织病理性改变明显减轻。结论rhKD/APP-var对大鼠慢性肝损伤具有明显的保护作用。

Objective To investigate the protective effect of the reorganization human Kunitz protease inhibitor domain of amyloid protein precursor variant （rhKD/APPvar）agaist chronic hepatic injury in rats.Methods The chroni hepatic injury model of rats were induced by carbon tetrachlofide （CCl4）.Measuring the changes of activity of serum alanine aminotransferase（ALT） ,aspartate amino- transferase （AST）, albumin（ ALB ）, albumin/globulin （A/G）, sialic acid （ SA ）, choline esterase （ CHE ）, alkaline phosphatase （ALP） and the hydroxyproline（Hyd） in the liver tissue, Observing the pathologic change of liver tissue. Results The rhKD/APPvar could decrease the concentration of serum ALT,AST,ALP and Hyol.The rhKD/APPvar could increase the concentration of serum ALB,A/ G,SA, CHE ignificantly. The rhKD/APPvar could improve the pathologic change of liver tissue of chroni hepatic injury significantly. Conclusion The rhKD/APPvar has protective functions on rats with chroni liver injury significantly