小鼠2种和人4种精子抗原免疫对小鼠妊娠的影响

Pregnance Status in Mice Immunized with Two Kinds of Mice and Four Kinds of Human Sperm Antigens

目的:观察用小鼠和人不同的精子抗原免疫雌小鼠对妊娠影响。方法:用小鼠2种和人4种精子抗原免疫雌小鼠,采用酶标法检测血清抗精子抗体(AsAb),观察各组小鼠妊娠率及胎仔数。结果:随着主动免疫的进行,小鼠血清AsAb显著升高(P<0.01)。精子抗原免疫小鼠8周后,小鼠精子混合抗原免疫小鼠效果最好,妊娠率为零,DTT提取的人精子膜抗原和人全精子抗原免疫小鼠效果较好,妊娠率为16.7%,显著低于正常对照组的63.6%(P<0.01或P<0.05)。结论:在人精子抗原中,DTT提取的人精子膜抗原和人全精子抗原免疫雌小鼠效果最好。在小鼠精子抗原中,DTT、NP-40、Triton-100提取的小鼠精子膜混合抗原免疫雌小鼠效果最好。

Objective：To observe the pregnance status of mice immunized with different kinds of mouse and human sperm antigens. Methods.. Female mice were immunized with different kinds of sperm antigens. Antisperm antibody（AsAb） in serum was detected by enzyme linked immunoabsorbant assay（ELISA）. The pregnance rate and the embryo number was computed. Results.. High level of AsAb was detected in serum after mice were immunized（P〈0.01）. The pregnance rate was zero in group G immunized with mixed mouse antigens, 16.7% in group B immunized with human sperm membrane antigen and group D immunized with human whole sperm antigen, being significantly lower than 63. 6% of group A injected with 9.0 g/L normal saline（P〈0.01 or P〈0.05）. Conclusion.. The pregnance rate was lowest when mice were immunized with membrane antigen of human sperm extracted with dithiothreitol（DTT） and whole human sperm antigen among different sperm antigen, and it was lowest when mice were immunized with mixed membrane antigen of mouse sperm extracted with DTT, NP-40 and Triton-100.