嗜碱性利用—碳的莫拉氏菌的研究

STUDIES OF AN ALKALOPHILIC C-1 COMPOUND ASSIMILA- TING BACTERIUM, MORAXELLA SP. M-z

利用不同营养类型的微生物进行CO_2固定的研究在世界上很受重视。对光能自养菌和化能自养菌;好氧菌和厌氧菌等多种类型的一碳微生物的比较生化学分析能使人们更好地认识它们潜在的应用价值。近年来,分离自极端环境微生物的CO_2固定研究已引起人们的极大兴趣。在pH9～10的偏碱性条件下,根据反应式(1)、(2)、(3),绝大部分CO_2以HCO_3^-和CO_3~2-;的形式存在。CO_2、H_2CO_3、HCO_3^-和CO_3~2-的总量多于中性环境的相应量。因此。

An alkalophilic C-1 compound assimilating bacterium, Moraxella sp. M-z, was used in this study. The optimum pH range of its growth is 8-10. Results showed this strain is a facultative C-1 utilizer, its growth rate could be raised by adding 0.01% yeast extract to the medium while formate or methanol was the sole carbon source, respectively. The specific activity of RubisCO were 0.14 U /mg · pr, 0.13 U /mg · pr and trace with those cell-free extract prepared from formate medium, methanol medium and LB medium. Formate dehydrogenase (DHE), formaldehyde DHE and methanol DHE which related with C-1 compound assimilation were also assayed.