纳米金表面二元自组装及其电泳表征

Self-Assembly of Mixed Monolayers on Gold Nanoparticles and Its Electrophoretic Characterazition

用DNA饱和包被纳米金,再用巯基己醇(MCH)和4-巯基苯甲酸(MBA)取代纳米金表面的DNA,在纳米金表面形成二元单分子层;用MBA饱和包被纳米金,用DNA取代纳米金表面的MBA,在纳米金表面形成二元单分子层;用凝胶电泳对二元单分子层的自组装过程进行表征。实验结果表明,含有MBA的二元单分子层能使纳米金稳定的分散于水溶液,而含MCH的二元单分子层中,MCH量过大时易使纳米金发生凝聚;在纳米金表面DNA取代MBA比MBA取代DNA更容易。

Mixed monolayer protected nucleic acid gold nanoprobes were prepared by modifying gold nanoparticles with both DNA and 4-mercaptobenzoic acid （MBA） or DNA and mercaptohexanol （MCH）. Agarose gel electrophoresis was used to characterize the reaction between DNA and MBA or DNA and MCH. The experimental results showed that MBA-DNA monolayers on gold nanoparticles was very stable while MCH-DNA monolayers on gold nanoparticles was apt to agglomeration when the quantity of MCH was very large, and DNA replacing MBA was easier than MBA replacing DNA.