十字花科蔬菜上黄瓜花叶病毒分离物的比较研究

COMPARATIVE STUDIES ON SIX CMV SOLATES FROM CRUCIFEROUS CROPS

对从杭州市郊青菜、萝卜、花椰菜上分离的6个CMV分离物进行了生物学、血清学及双链RNA比较研究。生物学测定结果表明,不同分离物在所测定的6种十字花科蔬菜上的致病力有差异。6种分离物颗粒形态、衣壳蛋白分子量和血清学方面无差异,且都属血清型Ⅰ。dsRNA分析结果表明,6个分离物的dsRNAl,2,3和4在PAGE中迁移率相似,但在1.5kb～0.4kb之间有多条量相对较少的条带,这些条带在不同分离物之间差异较大。6个分离物均含有卫星RNA。

Comparative studies were made among six CMV isolates collected from Brassica chinensis, Raphanus sativus and Brassica olemcea, which were named CMV B-931, B-932, R-931, R-932, C-931 and C-932, respectively. Biological tests showed the six isolates had different pathogenicity on six cruciferous crops. No difference was found in mophology of particles, molecular weight of coat protein and serology, and all the isolates belong to serotype I. Analysis of dsRNAs extracted from infected Nicotiana tabacum showed all the isolate had the some migration of each of the dsRNAl, 2, 3, 4. Many minor dsRNAs existed between 1.5kb and 0.5kb, and the profile of these dsRNAs were different between the isolates. All the isolates contain satellite RNA.