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血红素-琼脂糖修饰电极的电化学和电催化性质研究 被引量:5

The Electrochemistry and Electrochemical Catalysis of Hematin-agarose Modified Electrode
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摘要 用循环伏安法研究了血红素-琼脂糖凝胶修饰电极在水和有机溶剂/水混合溶液中的电化学和催化还原过氧化物的性质。包埋在琼脂糖水凝胶膜中的血红素能与电极之间直接传递电子,且具有过氧化物模拟酶的特性,能快速地催化还原过氧化物,可用于这些物质的定量检测。 Hematin (Hem) was immobilized on glassy carbon electrode (GCE) surface by agarose hydrogel. The fast and direct transfer-electron reactions both in aqueous and aqueous-organic solvents mixtures were investigated by cyclic voltammetry. E^0' is linearly dependent on solution pH, indicating the electron transfer of HemFe(Ⅲ)/( Ⅱ ) redox couple companied with the transfer of proton. The peroxidase-like activity for peoaises (hydrogen peroxide, Cumene hydroperoxide, t-butyl hydroperoxide and 2-butanone peroxide) of immobilized Hem was also explored. Stability and reproductivity of the modified electrode made it possible to determine these substances.
出处 《分析科学学报》 CAS CSCD 2008年第1期55-58,共4页 Journal of Analytical Science
基金 湖北省自然科学基金(No.2005ABA007) 湖北省教育厅科技项目(No.G200525001,T20030718)
关键词 血红素 电化学 过氧化物 模拟酶 Hematin Electrochemistry Peroxidase-like activity Peroxides
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