荧光分光光度法测定小诺霉素

Determination of Micronomicin by Fluorescence Spectrophotometry

小诺霉素具有微弱荧光，被高碘酸钠在沸水中氧化后水解形成醛基，可与乙酰丙酮及氨基在一定条件下生成一种具有强荧光的衍生物。此衍生物在常温下可稳定40min以上，其荧光强度较小诺霉素显著增强，且最大激发波长和发射波长分别红移33nm和68nm。在1．0×10^-8～1．0×10^-7mol／L浓度范围内，小诺霉素的荧光强度与其浓度呈良好的线性关系，相关系数r为0．9925。方法用于实际样品的测定，结果较为满意。

Micronomicin with weak fluorescence was oxidized to create active aldehyde groups with sodium metaperiodate, and further reacted with acetylacetone to produce a derivative with strong fluorescence. The fluorescence intensity of the derivative was stronger than that of micronomicin, and the maximum excitation and emission wavelengths were red shifted to 33 nm and 68 nm, respectively. A linear relationship between the concentration of micronomicin and the fluorescence intensity was obtained in the range of 1.0×10^- 8;1.0×10^- 7 mol/L with the detection limit of 6.7×10^-9 mol/L. The method was applied for the measurement of the content of micronomicin in real samples with satisfactory results.