摘要
采用反相高效液相色谱-质谱(HPLC-MS)联用技术分析了沙田柚果肉汁提取液中呋喃香豆素类化合物。沙田柚果肉汁用乙酸乙酯和正己烷的混合液(体积比5∶3)萃取,有机层经硅胶柱色谱分离后得若干个组分,用HPLC-MS对各组分进行定性鉴定。结果表明沙田柚果肉汁中含有6,7-二羟基香柠檬素(6,7-d ihydroxybergamottin,6,7-DHB)、香柠檬素(bergamottin)、5-甲氧基呋喃香豆素(bergapten)、5-甲氧基-8-异戊烯氧基呋喃香豆素(phellopterin)、6,7-环氧香柠檬素(6,7-epoxybergamottin)及二聚体对氧呋喃香豆素-6,7-二羟基香柠檬素(简称FC574)6种呋喃香豆素类化合物。并经HPLC-MS/MS进一步验证了其化合物结构。
A high performance liquid chromatography -mass spectrometry method was used for analysis and identification of furanocoumarins in pomelo juice extraction. Pomelo juice was extracted with ethyl acetate- hexane （5 ： 3 by volume）, separated by silica gel column chromatography into nine fractions, each fraction was analysed by HPLC - MS. Six furanocoumarins were identified in the pomelo juice and their structures were verified by HPLC - MS/MS.
出处
《分析测试学报》
CAS
CSCD
北大核心
2008年第2期170-173,共4页
Journal of Instrumental Analysis
基金
广东省食品工业公共实验室开放课题资助项目(FIPL-05-004)
