利用固定化酯酶制备(R)-氟比洛芬

Preparation of (R)-Flurbiprofen with Immobilized Esterase

本研究利用固定化重组酯酶由氟比洛芬乙酯拆分制备(R)-氟比洛芬。将基因工程菌经高压破壁的裂解液直接固定到含Ni2+的载体上,所得固定化酯酶的比活力为340u/g。反应液分离纯化后(R)-氟比洛芬的光学纯度达99.6%,收率为30.7%。

Immobilized esterase was used for the preparation of （R）-flurbiprofen. The cell wall of E.coli expressing esterase was broken by high pressure homogenization, and the lysate was directly immobilized to the carrier chelated with Ni^2＋ by affinity interaction. The specific activity of immobilized enzyme was 340u/g. After purification, the optical purity of （R） - flurbiprofen was 99.6 % with 30.7 % of total yield.