摘要
本研究利用固定化重组酯酶由氟比洛芬乙酯拆分制备(R)-氟比洛芬。将基因工程菌经高压破壁的裂解液直接固定到含Ni2+的载体上,所得固定化酯酶的比活力为340u/g。反应液分离纯化后(R)-氟比洛芬的光学纯度达99.6%,收率为30.7%。
Immobilized esterase was used for the preparation of (R)-flurbiprofen. The cell wall of E.coli expressing esterase was broken by high pressure homogenization, and the lysate was directly immobilized to the carrier chelated with Ni^2+ by affinity interaction. The specific activity of immobilized enzyme was 340u/g. After purification, the optical purity of (R) - flurbiprofen was 99.6 % with 30.7 % of total yield.
出处
《中国医药工业杂志》
CAS
CSCD
北大核心
2008年第2期99-100,112,共3页
Chinese Journal of Pharmaceuticals