黄芪中环阿屯烷型三萜皂苷的二维核磁共振研究

2D NMR study on cycloartane triterpenoids from Astragalus membranaceus var. mongholicus

目的:应用二维核磁共振技术(2D NMR)技术对蒙古黄芪[Astragalus membranaceus(Fisch.)Bge.var.mongholicus(Bge.)Hsiao]中环阿屯烷型四环三萜代表性化合物黄芪甲苷(astragaloside IV)的1HNMR和13C NMR信号进行准确归属,在此基础上,对其他6个环阿屯烷型皂苷的13C NMR信号进行准确归属。方法:运用2D NMR技术(1H-1HCOSY、HSQC、HMBC、TOCSY和ROESY实验)和选用高灵敏度低噪声的二维脉冲序列、梯度场技术。结果:首次准确地归属了7种环阿屯烷型四环三萜皂苷NMR谱中1H、13C信号的化学位移,并确定了其相对构型。结论:2D NMR是确认环阿屯烷型四环三萜皂苷NMR信号及相对构型的有效技术。

Aim：Using two-dimensional NMR （2D NMR） spectra to definitely assign the chemical shifts of ^1H and ^13C NMR of astragaloside IV and other cycloartane triterpenoids from Astragalus membranaceus （Fisch.） Bge. var. monghol/cus （Bge.） Hsiao.Methods：The 2D NMR technology （^1H-^1H COSY,HSQC,I-IMBC,TOCSY and ROESY） of high sensitivity and lower noise dimensional pulse sequences was used. Results：The ^1H and ^13C chemical shifts of the cycloartane triterpenoids have been definitely assigned by the application of 2D NMR. Conclusion：2D NMR is the stateof-art technology for the NMR signal assignment and the relative configuration determination of cycloartane triterpenoid.