重组人酸性成纤维细胞生长因子对创面愈合的作用

Effect of recombinant human acidic fibroblast growth factor on wound healing

目的:观察重组人酸性成纤维细胞生长因子(rhaFGF)对创面愈合的影响,探讨rhaFGF在组织修复中的作用机制。方法:利用大鼠创伤模型,采用HE染色观察不同时间点创面愈合情况;采用免疫组化方法检测伤后不同时间点创面愈合组织中半胱氨酸天冬氨酸蛋白酶(caspase-3)的表达情况;采用羟脯氨酸测试法检测伤口中胶原纤维的含量,并对照观察创面应用rhaFGF后以上各项指标的变化。结果:外用rhaFGF后创面组织中伤后第8天毛细血管胚芽与成纤维数量显著多于单纯创伤组,伤后第14天,经rhaFGF治疗的大鼠创面的再上皮化明显强于单纯创伤组。创面组织中caspase-3的表达量逐渐增加,外用rhaFGF后第14天和第21天的caspase-3表达量显著高于单纯创伤组。伤口中羟脯氨酸的含量逐渐增加,外用rhaFGF后第8天和第14天的羟脯氨酸含量显著高于单纯创伤组。结论:外用rhaFGF在创伤愈合的前期可以促进肉芽组织生长使得愈合周期缩短。在创伤愈合的后期可以直接或间接的促进成纤维细胞的凋亡,维持了细胞增殖与凋亡的平衡。

Aim：To observe the effect of recombinant human acidic fibroblast growth factor （rhaFGF） on wound healing, and to explore the mechanism related to the action of rhFGF. Methods： Rats of the wound skins were divided into two groups：injury group and rhaFGF-treatment group.At intervals of 4th, 8th, 14th, and 21st day, wound skins from ten rats were removed. The growth of wound granulation and re-epithelization were observed to evaluate the effect of rhaFGF. Immunohistochemical techniques were also employed to detect the expression of caspase-3 at the preplanned intervals. Then the L-Hyp method was Utilized to determine the content of collagen in the granulation tissues. Results： By the 8th day, fibroblasts and capillary granulation tissue in rhaFGF-treatment group grew more actively than the injury groups did. By the 14th day, re-epithelization in rhaFGF-treatment group was significantly more than in the injury group.The caspase-3 expression in dermal tissues increased gradually. By the 14th day and the 21st day, the caspase-3 expression in rhaFGF-treatment group was significantly more than in the injury group. The content of collagen in the granulation tissues increased gradually. By the 8th day and the 14th day, the content of collagen in rhaFGF-treatment group significantly more than in the injury group. Conclusion： rhaFGF could promote the growth of granulation tissue in the early phase of the wound healing, and directly or indirectly stimulate the apoptosis of cell in the late phase of wound healing.