毛细管气相色谱法测定辛夷药材中桉油精含量

Determination of Cineol in Xinyi by Capillary Gas Chromatography

目的建立辛夷药材中按油精含量的测定方法。方法采用水匀浆破碎，挥发油提取器中回流提取，FFAP毛细管色谱柱（30mm×0．32mm，0．32μm）分离，火焰离子化检测器（FID）检测，以苯乙烯为内标，毛细管气相色谱法测定辛夷药材中按油精的含量。结果在桉油精进样量为0．4～3．0mg时有良好的线性关系（r=0．9998）；加标平均回收率为99．3％，RSD为1．8％。结论加水匀浆破碎可有效地防止破碎过程中按油精的挥发。不同产地辛夷药材按油精含量相差很大。

Objective To develop a GC method for determination of cineol in Xinyi. Methods Xinyi was pulverized with water, extracted and separated with the capillary column FFAP （ 30 mm × 0.32 mm, 0.32 μm） and the content of cineol detected by flame ionization detector （FID）. Styrene was selected as the internal standard . Results The linear range of the method was 0.4 3.0 mg （r =0. 999 8） , the average recovery was 99.3% , RSD was 1.8%. Conclusion There exists considerable difference in the content of cineol in Xinyi, water stirring and pulverizing can avoid the volatility of cineol in the stirring process availably .