大豆苷元对神经母细胞瘤细胞增殖的抑制作用

The Inhibitory Effect of Daidzein on the Proliferation of Neuroblastoma Cells

目的探讨大豆苷元对神经母细胞瘤SH—SY5Y细胞凋亡和增殖的影响。方法采用细胞生长抑制实验（MTT法）、流式细胞仪检测等方法观察SH—SY5Y细胞的形态变化、细胞凋亡率及细胞周期变化。结果10．0～80．0μg／ml的大豆苷元明显抑制细胞的增殖，且有剂量依赖性；与对照组相比，大豆苷元处理24h后，各组SH—SY5Y细胞均出现亚二倍体峰（Sub—G1），细胞凋亡率与大豆苷元呈剂量依赖性。结论大豆苷元可诱导神经母细胞瘤SH—SY5Y细胞凋亡，对SH—SY5Y细胞增殖有明显的抑制作用。

Objective To observe the effect of Daidzein on neuroblastoma. Methods The proliferation was tested by MTT assay. Cell cycle and apoptosis rate were determined by flow cytometry. Results 10.0 - 80.0 μg/ml of Daidzein could significantly in hibit proliferation of SH -SYSY cell, Daidzein extract could induce apoptosis of SH -SYSY cell and the rate of apoptosis was in creased with the increase of the drug concentration. Conclusion Daidzein extract can induce apoptosis and inhibit proliferation of SH - SY5Y cell.