虎杖疏松愈伤组织诱导条件的研究

Study on the Loose Callus Induction of Polygonum cuspidatum

目的研究虎杖疏松愈伤组织诱导的最佳激素条件。方法通过单因素和正交实验研究不同外植体、不同浓度的激素组合对虎杖愈伤组织诱导率、疏松程度以及增殖率的影响。结果以诱导率为指标,NAA对虎杖细胞脱分化影响最显著。以增殖率为指标,2,4-D对虎杖的细胞生长分裂影响最显著。结论以叶柄为外植体更容易诱导出疏松的愈伤组织,虎杖愈伤组织诱导和继代增殖最佳的激素组合分别为6-BA 1.5 mg/L+2,4-D 1.0 mg/L+KT0.2 mg/L+NAA0.5 mg/L和6-BA2.0 mg/L+2,4-D 2.0 mg/L+KT0.4 mg/L+NAA0.4 mg/L。

Objective To study the optimum hormone factors of loose callus induction of Polygonum cuspidatum. Methods By single factor and orthogonal experiments, the effects of different explants and hormone combinations on callus induction , loosen and growth of Polygonum cuspidatum were investigated. Results NAA had remarkable effect on cell differentiation and 2,4 - D had remarkable effect on ceil division of Polygonum cuspidatum. Conclusion Leafstalk is much easier to loose callus in duction, the optimum hormone combinations of callus induction and growth of Polygonum cuspidatum are respectively 6 - BA 1. 5 mg/L ＋ 2,4 -D 1.0mg/L ＋KT0.2mg/L ＋NAA0.5 mg/L and6 -BA2.0 mg/L ＋2,4 -D2.0mg/L ＋KT0.4mg/L ＋NAA0.4 mg/ L.