脱除人主动脉瓣膜细胞的研究

Construction and evaluation of the property of decellular human aortic valve

目的探讨人主动脉瓣叶去细胞后作为组织工程心脏瓣膜支架的可行性。方法经胰酶-EDTA、表面活性剂和核酸酶处理,去除人主动脉瓣叶的细胞成分,测定瓣叶去细胞前、后的生物力学特性。结果人主动脉瓣去细胞后,经光镜和电镜观察,脱细胞后血管壁无细胞残留,胶原纤维和弹性纤维保留完整,形态学无明显变化;生物学性能也没有明显改变。结论去细胞效果良好,初步制造了同种主动脉血管壁脱细胞基质材料,为构建同种带瓣管道提供了较合适的材料。

Objective To construct decellular human aortic valve serving as a caffold for tissue engineering valve.Methods The porcine aortic valves were acellularized by use of trypsin-EDTA.TritonX-100,RNase and DNase treatment.Biomechanical characteristics of fresh valves and acellularized valve were tested.Results Light and electron microscopy confirmed that all the cellular constituents were removed without uhrastructural damage to fibrous component.Water content in decellular aortic wall was increased significantly;thickness and shrinkage emperature of the decellularized human homograft aortic had no significantly differences.Conclusion The method applied in this study could be effective according to the data. Meanwhile, the biological properties of the decellularized. Human homograft aortic valves were shown relatively stable. These valves might be used as an ideal valve for patients.