摘要
研究以陆海杂交棉为试材,研究了SSR检测体系中PCR扩增的各主要成分对检测结果的影响,确立了适宜的退火温度和反应体积,改进了PCR扩增程序,进一步优化了适用于棉花的SSR检测体系。同时,对聚丙烯酰胺凝胶银染程序作了调整,优化后的棉花SSR检测程序可得到条带强度适中、对比度好的结果。
The effects of main components of PCR reaction on SSR results were examined on upland and island hybrid cotton. The suitable anneling temperature and reaction volume were established. PCR procedure and silver staining method were improved, SSR detecting system on cotton was further optimized. The clear and reliable results can be abtained with the optimized dection procedures.
出处
《新疆农业科学》
CAS
CSCD
2008年第1期66-69,共4页
Xinjiang Agricultural Sciences
基金
新疆维吾尔自治区高技术研究发展计划项目(200311101)
关键词
棉花
SSR
聚丙烯凝胶电泳
cotton
SSR
Polyacrylamide gel electrophoresis
