微波消解-火焰原子吸收法测定乳汁中多种微量元素

Determination of trace elements in human milk by Flame Atomic Absorption Spectroscopy with Microwave Digestion

目的建立微波消解-火焰原子吸收法检测人乳汁中多种微量元素的技术方法并对其条件进行优化。方法应用具有压力控制功能的Multiwave 3000型微波样品制备系统，对产妇乳汁中铜、锌、钙、镁、铁和锰元素进行微波消解，并采用火焰原子吸收法测定其元素的含量。探讨了混合酸体系和微波消解时间对微波消解乳汁结果的影响。结果在混合酸体系HNO3-H2O2中，当混酸比为3：1、酸与样品体积比为4：3、每步微波消解时间为4min的条件下，消解效果最佳。在此条件下进行了火焰原子吸收测定消解产物的方法学评价。所得精密度试验中各元素相对标准偏差为3．13％～4．98％，回收试验的平均回收率在96．17％～103．38％。各元素在线性范围内相关性良好（r〉0．9998）。结论微波消解结合火焰原子吸收法测定母乳中微量元素，具有快速、简便、经济、准确等特点，适合临床检测。

Objective To establish a method of quantifying main trace elements in human milk by flame atomic absorption spectroscopy with microwave digestion. Methods A microwave digestion method was developed for the determination of Cu, Zn,Ca, Mg,Fe and Mn in human milk, using the Multiwave 3000 microwave sample preparation platform system, and these trace elements were quantified by flame atomic absorption spectrometry after microwave digestion. Then the digestion solution and digestion time were optimized before detection. Results We got satisfactory analysis results with the average recovery varied from 96.17% to 103.38% ,the CVs of imprecision were in the range of 3.13% -4.98% and the correlation coefficients of the linearity were over 0. 999 8 under the optimized analysis condition. Conclusion All these results prove this procedure to be a rapid,simple,economical and convenient method in quantifying trace elements in human milk.