鸡大肠杆菌病地方性致病菌株的分离鉴定及免疫研究

The Identification and Immunity of Pathogenetic E coil Isolated from Chicken in Jilin Province

在吉林省３个地区患大肠杆菌病的鸡肝脏、心血中分离出１１５株致病性大肠杆菌，并进行了生理、生化、血清学、免疫学及抗药性鉴定。吉林省地方株菌体抗原为Ｏ８８、Ｏ９、Ｏ２４、Ｏ６８、Ｏ５的菌株占５３％，能抵抗多种抗生素，以菌体抗原Ｏ２４、Ｏ６８、Ｏ８８和１种未能鉴别出血清型的菌株为代表菌株制成灭活水苗和油佐剂苗，用于免疫２５万只鸡，保护期６个月，保护率达９８％以上。

115 Ecoli strains isolated from liver and heart blood of chicken in 3 different areas of Jilin province were identified with physiological, biochemical, serological, immunological methods and tested with antibiotics The ratio of bacterial antigenicity of O88, O9,O24, O68,and O5 were 53% The bacteria can resist several kinds of antibiotics The unactive vaccine and vaccine of adjuvant were prepared with 4 tipical pathogenetic Ecoli strains After tested inspection of impurty, safety and effect, the vaccines were applied to 25000 chicken The protective ratio of the vaccines was as long as 6 months, the protective ratio of the vaccines was as high as 98% 