仔猪产肠毒素性大肠杆菌的血清学鉴定

Serological Identification of Piglets′ Enterotoxigenic E.coli

从吉林省某些猪场采集发生典型黄白痢的仔猪粪便样品５５份，采用ＥＭＢ选择培养基进行分离培养，得到４３株大肠杆菌，用Ｋ８８、Ｋ９９、９８７Ｐ３种因子血清分别进行玻片凝集试验。结果阳性菌株有２３株，其中１４株为Ｋ９９，占６０９％；５株为９８７Ｐ，占２１７％；３株含双粘附素（Ｋ９９、９８７Ｐ），占１３０％；只有１株为Ｋ８８，占４３％。

Fiftyfive piglets′ excrement samples of typical yellow diarrhea and white flux were gathered from some pigfarms in Jilin province.Adopting EMB culture medium to isolate and cultivate, 43 strains of E.coli were obtained. 3 monovalent serotypes,K99、K88、987P were conducted seperately in the plate agglutination test.23 strains are the positive species, and of which,14 strains are K99(609%);5 strains are 987P(217%);3 double adhesive antigen(130%);and only one is K88(43%).